Specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody

Immunofluorescent Staining: the Fluorescent Antibody Method.

I. Principles of Tmmunofluorescent Staining.................................................. A. Fluorescent Antibody Labeling........................................................ B. Immunofluorescent Staining Procedures............................................... C. Controls.............................................................................. D. Nonspecific Staining................

Papain-treated globulins in specific and cross-reacting immunofluorescent staining.

Jones, Wallis L. (Communicable Disease Center, Atlanta, Ga.), and J. W. Foster. Papain-treated globulins in specific and cross-reacting immunofluorescent staining. J. Bacteriol. 91:984-986. 1966.-Cross-reaction staining with preimmune and specific fluorescein conjugates to Corynebacterium diphtheriae was studied by use of papain-treated antisera. Enzyme-treated antisera were labeled and chromat...

Application of Specific Monoclonal Antibodies for Characterization of Leishmania spp. Promastigotes Using Indirect Immunofluorescent and Immunoperoxidase Tests

Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of   Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of   Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA).   Methods: Ap...

Successful Treatment of Refractory Thrombotic Thrombocytopenic Purpura with Rituximab, a Monoclonal Antibody: a Case Report

Thrombotic thrombocytopenic purpura (TTP) is mostly attributed to the presence of an autoantibody against ADAMTS-13, a metalloprotease that degrades ultralarge von Willebrand protein multimers. Accumulation of vWF multimers and systemic platelet aggregation lead to microangiopathic thrombosis, hemolytic anemia, and end-organ ischemia. Most patients respond to therapeutic plasma exchange (TPE), ...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody